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blasticidin s hci  (Tocris)


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    Structured Review

    Tocris blasticidin s hci
    Blasticidin S Hci, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blasticidin+s+hci/Blasticidin+S+HCl/10__1093_slash_bioadv_slash_vbaf063-303-10-14
    Average 93 stars, based on 5 article reviews
    blasticidin s hci - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/well density and allowed to adhere and form monolayer in growth media.

    Article Title: A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/ well density and allowed to adhere and form monolayer in growth media.

    Article Title: [2401.02501] A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/ well density and allowed to adhere and form monolayer in growth media.

    Expressing:

    Article Title: A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/well density and allowed to adhere and form monolayer in growth media.

    Article Title: A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/ well density and allowed to adhere and form monolayer in growth media.

    Article Title: [2401.02501] A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/ well density and allowed to adhere and form monolayer in growth media.

    Imaging:

    Article Title: A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/well density and allowed to adhere and form monolayer in growth media.

    Article Title: A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/ well density and allowed to adhere and form monolayer in growth media.

    Article Title: [2401.02501] A Kolmogorov metric embedding for live cell microscopy signaling patterns
    Article Snippet: To generate cell lines stably expressing nuclear marker and biosensors, transfection was carried out with FuGene (Promega). .. Stable clones expressing biosensors were selected using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc-29067, Lab Force) and imaging-based screening. .. MCF10A cells and knock-in/out derivatives were plated on Fibronectin (PanReac AppliChem) coated (0.25ug/cm2) 96 well 1.5 glass bottom plates (Cellvis) at 30 000 cells/ well density and allowed to adhere and form monolayer in growth media.

    Selection:

    Article Title: Optogenetic actuator – ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics
    Article Snippet: Chemical perturbations were done with SU‐5402 (SML0443, Sigma), RAF709 (HY‐100510, Lucerna‐Chem), U0126 (S1102, Selleck chemicals, Lubio), SCH772984 (HY‐50846, Lucerna‐Chem), SL0101 (559285, Sigma), Cyclosporine A (10‐1119, Lucerna‐Chem), and Ionomycin (sc‐3592, Santa Cruz). .. Selection of the cells post transfection was done using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc‐29067, Lab Force). .. The optoFGFR construct was a gift from Won Do Heo (Addgene plasmid # 59776) (Kim et al , ).

    Article Title: Optogenetic actuator/biosensor circuits for large-scale interrogation of ERK dynamics identify sources of MAPK signaling robustness
    Article Snippet: Chemical perturbations were done with SU-5402 (SML0443, Sigma), RAF709 (HY-100510, Lucerna Chem), U0126 (S1102, Selleck chemicals, Lubio), SCH772984 (HY-50846, Lucerna-Chem), SL0101 (559285, Sigma), Cyclosporine A (10-1119, Lucerna-chem) and Ionomycin (sc-3592, Santa Cruz). .. Selection of the cells post transfection was done using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris) and Hygromycin B (sc-29067, Lab Force). .. The optoFGFR construct was a gift from Won Do Heo (Addgene plasmid # 59776) ( ).

    Transfection:

    Article Title: Optogenetic actuator – ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics
    Article Snippet: Chemical perturbations were done with SU‐5402 (SML0443, Sigma), RAF709 (HY‐100510, Lucerna‐Chem), U0126 (S1102, Selleck chemicals, Lubio), SCH772984 (HY‐50846, Lucerna‐Chem), SL0101 (559285, Sigma), Cyclosporine A (10‐1119, Lucerna‐Chem), and Ionomycin (sc‐3592, Santa Cruz). .. Selection of the cells post transfection was done using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris), and Hygromycin B (sc‐29067, Lab Force). .. The optoFGFR construct was a gift from Won Do Heo (Addgene plasmid # 59776) (Kim et al , ).

    Article Title: Optogenetic actuator/biosensor circuits for large-scale interrogation of ERK dynamics identify sources of MAPK signaling robustness
    Article Snippet: Chemical perturbations were done with SU-5402 (SML0443, Sigma), RAF709 (HY-100510, Lucerna Chem), U0126 (S1102, Selleck chemicals, Lubio), SCH772984 (HY-50846, Lucerna-Chem), SL0101 (559285, Sigma), Cyclosporine A (10-1119, Lucerna-chem) and Ionomycin (sc-3592, Santa Cruz). .. Selection of the cells post transfection was done using Puromycin (P7255, Sigma), Blasticidin S HCI (5502, Tocris) and Hygromycin B (sc-29067, Lab Force). .. The optoFGFR construct was a gift from Won Do Heo (Addgene plasmid # 59776) ( ).



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